张玲莉¹  彭燕¹ 涂毅² （1 武汉大学人民医院药学部 430060 2  武汉大学人民医院普外科   430060 ）

[摘 要]  目的：用高效液相色谱法测定黄芩口服液中黄芩苷的含量。方法：色谱柱为ODS C₁₈（4.6mm×250mm，4µm），流动相为甲醇－水－磷酸（40:60: 0.2），检测波长280nm。结果：黄芩苷在0.813～4.065µg范围内进样量与峰面积呈现良好的线性关系（r＝0.9999）；平均加样回收率为98.6％，RSD＝2.4％（n＝5）。结论：本方法测定结果准确，重现性好。

[关键词]  高效液相色谱法； 黄芩口服液； 黄芩苷 ；含量测定

Determination of Content of Baicalin in HuangQin Oral by HPLC

Zhanglingli ，Peng Yan， Tuyi（The People's Hospital of WuHan University, 430060, P. R. China）

ABS.TRACT Objective: To develp a HPLC method for determination of the content of baicalin in HuangQin Oral. Method: ODSC18 column was used and methanol-water-phosphoric acid（40:60: 0.2）was used as mobil phase and UV detection wavelength was 280nm.  Result: There was a good liner relationship between the concentration of baicalin and peak area in with range of  0.813～4.065µg r＝0.9997.The average recovery was 98.6％ with RSD＝2.4％（n＝5）. Conclusion: The method is higyly repeatable and accurate.