高效液相色谱法测定病毒清口服液中 绿原酸的含量 李忠(郴州监狱职工医院,湖南,423000) 【摘要】 目的 建立病毒清口服液中绿原酸的含量测定方法。方法 色谱柱:Lichrospher C18(4.6*200mm,5um),流动相:甲醇:0.2mol/L磷酸二氢钠(用磷酸调节PH至3.2)(25:75),流速0.8ml/min,检测波长327nm。结果 绿原酸在7.5---37.5ug范围内呈现良好的线性关系,r=0.9996,平均回收率为97.64%,RSD=1.2%。结论 本方法简便、快速、准确,分离效果好。 【关键词】 病毒清口服液;HPLC法;绿原酸 Determination of chlorogenic acid in Bingduqing mixture by HPLC Lizhong (Staff workers’hospital of Chenzhou’s prison, Hunan,423000) 【Abstract】 Objective To establish a simple sensitive HPLC method for determining chlorogenic acid in Bingduqing mixture. Methods Chlorogenic acid was separated on Lichrospher C18 column(4.6*200mm,5um)with a mobile phase of methanol:0.2mol/L sodium dihy-drogen phosphate (regulate acidity to 3.2 use phosphoric acid) (25:75) and detected at 327nm with a flow rate of 0.8ml/min. Results The linear anger was 7.5--37.5ug, r=0.9996. The average recovery was 98.19%,RSD=1.3%。 Conclusions The method is simple ,rapid and reliable ,good separating degree. 【Key words】 Bingduqing mixture;HPLC;chlorogenic acid资料来自:中国药师-专业站 |
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