| ELISA法与MEIA法快速定量检测肝移植患者乙肝免疫球蛋白滴度
张弋1、窦志高2(1.天津市第一中心医院,天津 300192;2.天津医科大学药学院实习生,天津300070)
摘要 目的：比较微粒子酶免疫免疫法（MEIA）和酶联免疫法（ELISA）检测肝移植患者乙肝免疫球蛋白滴度（HBIG）的特点，总结其在临床HBIG滴度检测中的应用。方法：分别以MEIA和ELISA平行测定50、100、500IU.L-1的HBIG标准品，比较两种方法的准确度、精密度、相关性及各自特点。结果：MEIA和ELISA方法学的回收率分别为(105.32±1.85)%和(103.71±3.76)%,RSD分别为(3.67±2.30)%和(2.75±0.74)%,r=0.784。检测HBIG滴度≤160IU.L-1时,两个方法的相关性为MEIA=0.7095ELISA+30.1423,r=0.784(n=26)。当HBIG滴度〉160IU.L-1时，两方法检测结果MEIA=-0.010ELISA+159.197,r=0.032(n=29)。结论：ELISA法可用于常规的HBIG检测，而MEIA的检测范围比较宽，不仅可用于常规HBIG的检测，更适合HBIG被动免疫后人群的临床监测。
关键词 微粒子酶免疫法；酶联免疫法；乙肝免疫球蛋白；治疗药物监测
Comparison of MEIA and ELISA for the determination of hepatitis B immunoglobulin in whole blood samples in liver transplantation recipients
Zhang Yi，Dou Zhigao(Tianjin First Central Hospital,Tianjin 300192;Tianjin Medical University,Tianjin 300070)
ABSTRACT OBJECTIVE To compare MEIA assay and ELISA for the determination of hepatitis B immunoglobulin in liver transplantation recipients,and sum up its clinical application in organ transplantion.METHODS Recoveries of two methods were calculated by detemiming different level of HBIG standard in whole blood samples.RESULTS The recovery of the MEIA and ELISA were (105.32±1.85)% and (103.71±3.76)% respectively.The RSD of the MEIA and ELISA were (3.67±2.30)% and (2.75±0.74)% respectively.CONCLUSIONS Both methods are suitable for TDM in hospital.
KEY WORDS:MEIA;ELISA;HBIG;TDM
资料来自:中国药师-专业站 |
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