郭辉 张红旭 王慧1 吕冬梅2
江苏省徐州市第三人民医院徐州 221005
摘要 目的：用高效凝胶色谱法测定红毛五加多糖分子量。方法色谱柱为OHPaK SB-805HQ（8mm*300mm），以高纯水为流动相，流速0.8ml.min-1，柱温35度，示差折光器检测。结果：AGP1 分子量为91 000；AGP2分子量为85 000；AGP3分子量为45 000。结论：红毛五加多糖分子量在91 000～45 000之间,由AGP1，AGP2，AGP3三种组分组成。
关键词 高效凝胶色谱法，薄层色谱法，红毛五加多糖
Determination of Molecule Weight of Polysaccharrides of Acanthopanax giraldii Harms by HPGPC
Guo Hui, ZhangHongxu, Wang Hui , Lu Dongmei
The Third people,s hospital of Xuzhou city ,Jiangsu 221005,P.R.China
Abstract Objective: To determine the Molecule weight of Polysaccharride of Acanthopanax giraldii Harms by HPGPC. Methods: （1）The Molecule weights of Polysaccharrides of Acanthopanax giraldii Harms were determined by using HPGPC.（2）The components of hydrolysates of Polysaccharrides of Acanthopanax giraldii Harms was analyzed by using TLC. Result : The Molecule weights of AGP1 ,AGP2,AGP3 were 91000 、85000 and 45000 , respectively . The components of hydrolysates of AGP1 ,AGP2,AGP3 were D-galacturonicacid,galactose,arabinose and an unknown monosaccharide; D-galacturonicacid , galactose , arabinose , xylose,rhamnose and an unknown monosaccharide ; galactose,arabinose , xylose, rhamnose and an unknown monosaccharide, respectively . Conclusion: The Molecule weights of Polysaccharrides of Acanthopanax giraldii Harms was between 91000 and 45000, and it composed by AGP1,AGP2,AGP3.
Key words HPGPC ,TLC , Polysaccharride, hydrolysates
资料来自:中国药师-专业站 |