黄传俊 ¹ 李其兰¹ 高申蓉¹ 陈红专 ²( 1  湖北省中医院  武汉  430061  2  湖北中医学院2001级研究生 武汉 430061)

摘要：目的 优选黄连最佳提取工艺。方法 采用正交试验法，以黄连提取的收膏率和盐酸小檗碱提取量为考察指标，利用HPLC法测定盐酸小檗碱的含量。结果:最佳提取工艺为黄连粗粉加8倍量60℅的乙醇回流提取3次，每次1小时。结论：优选得到的工艺简便易行，稳定性好。

关键词：黄连﹔盐酸小檗碱﹔提取工艺﹔正交试验

Optimization of Extraction Process of Rhizoma Coptis with Orthogonal Design Method

Huang Chuanjun ¹, Li Qilan ¹, Gao Shenrong ¹, Chen Hongzhuan ²﹝1.HuBei Hospital of Traditional Chinese Medicine, WuHan,  430061  2.HuBei College of Traditional Chinese Medicine WuHan, 430061)

ABSTRACT Objective: To optimize the extraction process of Rhizoma Coptis. Method: Using orthogonal design method, to apply extract yield of Rhizoma Coptis and the extract amount of berberine hydrochloride as observing indexes, to measure the content of berberine hydrochloride with HPLC. Result: The optimum extraction process was as follows, adding 8 times of 60％alcohol into coptis meal, refluxing and extracting for 3 times ,one hour every time. Conclusion: This optimized process is simple, stable and efficient.

KEY WORDS Rhizoma Coptis; Berberine hydrochloride; Extraction process; Orthogonal design.