杨   珺 王世岭 郭晓华 左秀平  吴  坤 (军事医学科学院附属医院药剂科，北京市  100039)  

摘  要  目的：应用高效液相色谱法测定复方苦参凝胶剂中辣椒碱与苦参碱的含量。方法：采用Waters μBONDAPAK C18柱（3.9 μm×300mm），乙腈-0.05mol/L磷酸二氢钠溶液-三乙胺（50：50：0.05）；用磷酸调整PH值至2.2为流动相；检测波长为227nm；流速：1.0ml/min。结果：辣椒碱线性范围为6.25～200μg/ml (r=0.9999  n=5)；苦参碱线性范围为80.0～800.0μg/ml (r=0.9997   n=5)。辣椒碱平均回收率为100.40%～103.73%（n=9），RSD=1.54%；苦参碱平均回收率为100.21%～100.81%（n=9），RSD=1.12%。结论：本方法用于凝胶剂中辣椒碱与苦参碱含量的测定简便、快速、灵敏、结果准确。

关键词：反相高效液相色谱法；苦参碱；辣椒碱；含量测定

Determination of the Content of Capsaicin and Matrine in Hydrogel by HPLC

Yang Jun, Wang Shiling, Pan Qiang(Dept of Pharmacy, Hospital Affiliated to the Academy of Military Sciences, Beijing 100039, China)

ABSTRACT Objective: To establish a HPLC method to determine the content of capsaicin and matrine hydrogel. Method: The μ BONDAPAK C18 column was used, the mobile phase was acetonitrile solution—triethylamine (50:50:0.05).  Adjust pH to 2.2 with phosphorie acid_. The flow rate was 1.0ml·min^-1. The detection wavelength was at 227nm. Result: The linear range of capsaicin and  matrine were 6.25～200μg·ml^-1 (r=0.9999  n=5) and 80.0～800.0μg·ml^-1 (r=0.9997 n=5) respectively. 10; The average recoveries were 0.40%～103.73% （n=9, RSD=1.54%） and 100.21%～100.81%（n=9, RSD=1.12%）. Conclusion: The method is accurate, rapid and simple.

KEY WORDS RP-HPLC; Matrine; Capsaicin; Determination of the content