杨宏健1 肖美凤1 袁丽2 戴云花2 何迎春2
（1.怀化医学高等专科学校 天然药物化学教研室 湖南 怀化 418000
2. 怀化医学高等专科学校 药学专业02届学生 湖南 怀化 418000)
摘要：目的：优选三颗针中小檗碱的提取工艺。方法 ：采用正交试验设计法考察4个因素对盐酸小檗碱得率的影响。 结果：优选出最佳提取工艺为：A1B3C1D3即稀硫酸浓度0.2%，溶剂的量为16倍，石灰乳调ＰH9，精制时调酸的温度为600C。结论：以该工艺从三颗针中提取的小檗碱含量高，工艺较简单、适用。
关键词：三颗针 盐酸小檗碱 正交实验
中图分类号：R284.2 文献标识码：
Optimization of Extraction Technology of Berberine Hydrochloride in Berberis with Orthogonal Test
Yang Hongjian1, Xiao Meifeng1, Yuan Li 2, Dai Yunhua 2, He Yingchun2(1. Huaihua Medical College of Hunan province, Huaihua, 418000; 2. Grade 2 of Dept of Pharmacy)
ABSTRACT Objective: To optimize the extraction technology of berberine from berberis. Method: The orthogonal test method was adopted to explore the influences of 4 factors on the yielding rate of berberine from berberis. Result: The optimum conditions of extraction process were as follows： A1B3C1D3.The conditions was 0.2% H2SO4 with 16 times of solvent, lime emulsion for pH9．The temperature was about 60 oC. Conclusion: With the extraction technology, the berberine goods are of high content. The technology was simple and practical．
KEY WORDS: Berberis; Berberine hydrochloride; Orthogonal test
资料来自:中国药师-专业站 |