李志勤 （华中科技大学同济医学院附属同济医院药学部 武汉 430030） 鞠文（华中科技大学同济医学院附属协和医院泌尿外科 武汉 430022）
摘 要 目的: 建立测定血清中更昔洛韦的RP-HPLC法。方法：以阿昔洛韦为内标，采用C18柱及保护柱，流动相为甲醇－水（60：40，v/v），磷酸调PH6.5。流速1.0ml.min-1。紫外检测波长254nm。结果：更昔洛韦与内标分离良好，更昔洛韦保留时间9.25min,阿昔洛韦保留时间为11.23min。回归方程为A＝1.079×102C－0.202 (r=0.9997)。其线性范围为0.05―10µg.ml-1。结论：该法操作简便，测定结果精密度高，重现性理想，适用于药动学研究。
关键词 更昔洛韦 血药浓度 反相高效液相色谱法
Reversed-Phase High-performance Liqiud Chromatographic Assay of Ganciclovir in Human Serum
Li Zhiqin, Ju Wen (Dept of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030)
ABSTRACT Objective: To set up reversed-phase high performance liquid chromatographic assay to determine the content of Ganciclovir in human serum. Method: Aciclovir was used as internal standard. Analysis was carried out on a chromatographic system using C18 column and UV detection at 254 nm. Methanol-water (60:40) was used as mobile phase, adjusted pH6.5 with phosphoric acid. Velocity of flow was 1.0 ml.min-1. Result: The Ganciclovir and internal standard departed well. The retention time of Ganciclovir and aciclovir were 9.25 min and 11.23 min. The regress equation was as follows: A＝107.9C－0.202 (r=0.999 7). Calibration was linear from 0.05 to 10 µg.ml-1. Conclusion: This assay is easy to operate and to be repeated. It appears to be suitable for the pharmacokinetic studies.
KEY WORDS Ganciclovir; Blood drug concentration; RP-HPLC 资料来自:中国药师-专业站 |