黄娟 韩敏 刘慎微 刘晓城 徐钢 （华中科技大学同济医学院附属同济医院 武汉 430030）
摘 要 目的：观察缬沙坦对实验性2 型糖尿病大鼠肾脏病变的保护作用及其机制。方法：链脲佐菌素（STZ）诱导的糖尿病肾病大鼠模型随机分为对照组糖尿病组及治疗组（缬沙坦10 mg·kg-1·d-1），治疗6周后,比较各组大鼠肾组织中丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性，同时检测各组大鼠血糖、血肌酐、尿素氮、24 h 尿蛋白定量等。采用RT-PCR方法检测P22phoxmRNA表达。结果：治疗组较非治疗组明显改善尿白蛋白、尿素氮水平和肾脏肥大指数。肾脏MDA含量明显下降（P＜0.05＝。SOD活性显著上升（P＜0.05）。p22phox 的mRNA 相对含量在糖尿病组为（0.875±0.94），明显高于单切对照组（0.297±0.067）（P＜0.01），在治疗组为（0.484±0.064），较糖尿病组显著降低（P＜0.01），但仍高于对照组（P＜0.05）。结论：缬沙坦对2 型糖尿病肾脏病变有一定的保护作用,其机制可能是通过抑制氧化应激反应，下调糖尿病大鼠P22phoxmRNA的表达对2型糖尿病模型大鼠肾脏产生保护作用。
关键词 缬沙坦；2型糖尿病；NADPH氧化酶；糖尿病肾病
Mechanic Effect of Valsartan on Antioxidative Stress of Kedney in Type 2 Diabetic Rats
Huang Juan，Han Min，Liu Shenwei，Liu Xiaocheng，Xu Gang（Tongji Medical College Affiliated Tongji Hospital，Huazhong University of Science and Technology，Wuhan 430030，China）
ABSTRACT Objective：To investigate the effects of valsartan on renal lesions in type 2 diabetic rats。Method：Streptozotocin induced diabetic rats were randomly assigned to three groups：control rats，type 2 diabetic rats and type 2 diabetic rats treated with Valsartan（10 mg·kg-1·d-1）。After 6 weeks，content of MDA and activity of antioxidant enzymes including SOD in the renal tissue were compared among the groups。In addition，BG，Scr，BUN and urinary protein were measured respectively。P22phox gene expression was studied with a RT-PCR technique。Result：Compared with the untreated group，the level of BUN，Scr，renal MDA in Valsartan treated group were significantly decreased，and the activity of renal SOD were increased apparently。NADPH oxidatse subunit p22phox mRNA was significantly increased in the kidney of diabetic rats。Conclusion：Valsartan can amelibrate the renallesion in type 2 diabetic rats，antioxidation and inbibition of pzzphox-mRNA maybe the possible mechanism on nephro-protective action。
KEY WORDS Valsartan；Type 2 diabetes mellitus；NADPH oxidatse；Diabetic nephropathies 资料来自:中国药师-专业站 |