吴星火 聂宇 杨述华 王晶 夏天 杨操
(华中科技大学同济医学院附属协和医院骨科 武汉 430022)
摘 要 目的：探讨胰岛素对大鼠急性脊髓损伤的保护作用及其效果。方法：采用改良Allen WD 法建立大鼠急性脊髓损伤模型。60只SD大鼠随机分为损伤对照组和胰岛素治疗组，术后8 h, 1, 3, 7,14 d检测大鼠脊髓损伤后运动诱发电位（MEP）的潜伏期及波幅；采用原位末端标记（TUNEL）观察脊髓细胞凋亡；采用激光多普勒血流仪(LDF) 监测了胰岛素对伤段脊髓SCBF 量的影响。结果：术后各时相胰岛素组MEP潜伏期与对照组相比无明显差异，MEP波幅较对照组明显增高（P<0.05）；胰岛素治疗组TUNEL 阳性细胞的数目明显少于损伤对照组(P<0.05)；两组脊髓血流量（SCBF）均明显下降，但对照组下降更明显。结论：初步研究表明胰岛素能改善脊髓血流量、抑制神经细胞的凋亡、减轻神经功能缺失，对急性脊髓损伤有保护作用。
关键词 胰岛素；脊髓损伤；大鼠；实验研究
中图分类号：R965.1
文献标识码：A
文章编号：1008-049X（2007）02-0122-03
Experimental Study of the Neuroprotective Effect of Insulin to Acute Spinal Cord Injury in Rats
Wu Xinghuo，Nie Yu，Yang Shuhua，Wang Jing，Xia Tian，Yang Cao（Department of Orthopedics，Union Hospital，Tongji Medical College of Huazhong University of Science and Technology，Wuhan 430022，China）
ABSTRACT Objective：To study the protective effect of insulin to acute spinal cord injury in rats。Method：Acute spinal cord injury model in rats was built by modified Allen WD。Sixty healthy adult Sprague-Dawley rats were randomly divided into two groups：control group and insulin group。The latency and amplitude of MEP（Motion Evoked Potential）were analyzed at 8 h，1 d，3 d，7d，14 d postoperatively （n=6 in each time point） were analyzed。Apoptosis neurons were labeled with TUNEL dyeing。The spinal cord blood flow（SCBF）was recorded by laserDoppler flowmetry。Result：There were no changes in the latencies of MEPs in both groups，and the mean amplitudes of MEPs were higher in insulin groups than in control groups。The number of TUNEL staining positive cells in the insulin group was less than in control group（P<0.05）。And SCBF decreased in both groups，especially in control group。Conclusion：The study indicated that insulin could improve SCBF，inhibit neurocyte apoptosis，palliate loss of neural function，which might protect injured spinal cord。
KEY WORDS Insulin；Spinal cord injury；Rat；Experimental study
作者简介：吴星火，男，博士在读，主要从事骨组织工程的研究。
E-mail：wuxinghuo@163.com
作者简介：杨述华，教授，博士生导师，主要从事骨与关节疾病研究。
Tel：（027）85726196
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