尹雄章黄泽中 (华中科技大学同济医学院附属同济医院药学部 武汉 430030)
宋娅玲(武钢职工总医院中医科)
摘 要 目的：建立一枝黄花中槲皮素的含量测定方法。方法：采用HiQ sil C18色谱柱（250 mm×4.6mm，5μm）；柱温：40 ℃；流动相：甲醇-0.1％磷酸溶液（50∶50）；流速：1.0ml·min-1；检测波长：371 nm。结果：槲皮素进样量在0.40～4.04μg范围内线性关系良好（r＝0.999 7），平均回收率为100.2%；RSD为1.7％（n＝9）。结论：该含量测定方法简便准确。
关键词 一枝黄花；槲皮素；高效液相色谱法
中图分类号：R927.2
文献标识码：A
文章编号：1008-049X(2007)03-0259-02
Determination the Content of Quercetin in Herba Solidaginis by RP-HPLC
Yin Xiongzhang, Huang Zezhong (Department of Pharmacy, Affiliated Tongji Hospital，
Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030,China)
Song Yaling(Worker's General Hospital of Wuhan SteelIron Company)
ABSTRACT Objective: To establish a RP-HPLC method for determination the content of quercetin in herba solidaginis. Method: HiQ sil C18 column（250 mm×4.6mm5μm）was used, the column temperature at 40 ℃, the methanol-0.1% phosphoric acid solution (50∶50) as mobilephase. The flow rate was 1.0 ml·min-1 and the detection wavelength was 371 nm.Result: The linear range of quercetin was 0.40-4.04μg（r＝0.999 7）.The average recovery was 100.2％with RSD 1.7％（n＝9）.Conclusion: The method is simple and accurate.
KEY WORDS Herba solidaginis; Quercetin; HPLC
作者简介：尹雄章，男，药师，主要从事医院中药学工作。
Tel:(027)83663558
参 考 文 献
1中国药典［S］.1977年版.一部.1
2国家中医药管理局《中华本草》编委会.中华本草(精选本)下册［M］.上海：上海科学技术出版社，1996.1971 资料来自:中国药师-专业站 |