付萍萍1 杨开宁2 赵志伟1 景浩然1 (保定市药品检验所 河北保定 071051；2.保定市第二中心医院)
摘 要 目的：建立HPLC法测定烟酸维生素B6注射液中烟酸和维生素B6含量的方法。方法：采用C18 (200 mm×4.6mm，5μm) 色谱柱，以0.04%戊烷磺酸钠溶液（用冰醋酸调节pH为3.0±0.05）甲醇（90∶10）为流动相，检测波长为262 nm，流速为1.0 ml• min-1。结果：烟酸的线性范围为0.04～1.01μg（r=1.000 0，n=7）,平均回收率为100.3%（n=9）,RSD为0.4%；维生素B6的线性范围为0.12～3.03μg（r=1.000 0，n=7）,平均回收率为100.2%（n=9）,RSD为0.5%。结论：本方法快速、准确，可用于烟酸维生素B6注射液的质量控制。
关键词 烟酸；维生素B6；含量测定; 高效液相色谱法
中图分类号：R927.2
文献标识码：A
文章编号：1008-049X(2007)06-0551-02
Determination the Contents of Two Components in Nicotinic Acid and Vitamin B6 Injection by HPLC
Fu Pingping1，Yang Kaining2，Zhao Zhiwei1，Jing Haoran1(Baoding Institute for Drug Control， Hebei，Baoding 071051 China；2.Baoding Second Central Hospital)
ABSTRACT Objective: To establish a RPHPLC method for determination the contents of nicotinic acid and Vitamin B6 in Nicotinic acid and Vitamin B6 injection. Method: The Hypersil C18column(200 mm×4.6 mm,5μm)was used,the 0.04%Sodium Pentanesulfonate solution（adjuste pH to 3.0±0.05 with glacial acetic acid）methanol（90∶10）as the mobile phase,the flow rate was 1.0 ml•min-1.The detection wavelength was at 262 nm.Result: The linear ranges of nicotinic acid and vitamin B6 were at 0.04-10 μg（r=1.000 0）and 0.12-3.03 μg（r=1.000 0）respectively,the average recoveries were 100.3% and 100.2%with RSD 0.4% and 0.5%respectively.Conclusion: The method is quick and accurate.It can be used for quality control of nicotinic acid and vitamin B6 injection.
KEY WORDS Nicotinic Acid; Vitamin B6；Content determination; HPLC
参 考 文 献
1 中国药典［S］.2005年版. 二部. 667 资料来自:中国药师-专业站 |