王晓娟 王荣 高迎春 邓翀 (第四军医大学口腔医学院药剂科 西安 710032) 李骁勇 赵新峰 (西安交通大学中草药现代化研究工程中心）
摘 要 目的：建立反相高效液相色谱法测定人血浆中盐酸伪麻黄碱浓度的方法。方法：采用Dikma C18色谱柱（250 mm×4.6 mm，5 μm），流动相：乙腈-水（含0.2％三乙胺）＝10∶90（磷酸调pH3.3）；检测波长：195 nm，流速：0.8 ml•min-1。结果：盐酸伪麻黄碱浓度在20～800ng•ml-1范围内线性关系良好，最低定量浓度为20 ng•ml-1，提取回收率在59.65%～72.20%之间，日内和日间RSD分别为 1.34%～4.11%和 7.92%～9.98%。结论：本法操作简便、快速、准确、重现好，适用于盐酸伪麻黄碱的药动学研究。
关键词 盐酸伪麻黄碱；反相高效液相色谱法；血药浓度
中图分类号：R97
文献标识码：A
文章编号：1008-049X(2007)06-0550-02
Determination of Pseudoephedrine in Serum by RP-HPLC
Wang Xiaojuan,Wang Rong，Gao Yingchuen，Deng Chong（Department of Pharmacy,Stomatological College, Fourth Military Medical University, Xi'an 710032,China）；Li Xiaoyong，Zhao Xinfeng（School of Science of Xi'an Jiaotong University）
ABSTRACT Objective: To establish a RPHPLC method for determination the concentration of pseudoephedrine in plasma.Method: The separation of pseudoephedrine was performed on Dikma C18column (250 mm×4.6 mm,5μm).The mobile phase was composed of 0.2 triethanolamine solution acetonitrile=90∶10（pH=3.3）.The detection wavelength was 195nm and the flow rate was 0.8ml•min-1.Result: The linear range of pseudoephedrine was 20-800 ng•ml-1，The minimal detectable concentration in plasma was 20 ng•ml-1，the recovery rate was 59.65%-72.20% with RSD at1.34%-4.11% intraday and RSDat 7.92%-9.98%interday.Conclusion: This method is simple，fast，accurate and reproducible，which can be used as the pharmacokinetic study for pseudoephedrine in human body.
KEY WORDS Pseudoephedrine；RP-HPLC；Blood concentration
参 考 文 献
1 周松，陈腾．盐酸伪麻黄碱缓释制剂的研制及其体外释放度测定［J］．中国药师，2006，9（8）：734-736
2 周征．用HPLC法测定双扑伪麻片中盐酸伪麻黄碱的含量［J］．中国药师，2001，4（3）：204-205 资料来自:中国药师-专业站 |