徐帆 冯恩富 余昉 (成都军区昆明总医院 昆明 650032)
摘 要 目的：建立高效液相色谱法测定血浆中非诺贝特活性代谢产物非诺贝特酸浓度。方法：以甲醇直接沉淀血浆蛋白, 色谱柱为Waters sunfire C18柱（150 mm×4.6 mm，5 μm），流动相为0.05 mol•L-1磷酸二氢钾溶液-甲醇（70∶30），用磷酸调pH2.5,检测波长286 nm。结果：非诺贝酸的保留时间约为6.7 min,线性范围为0.2～20.0 μg•ml-1 ( r=0. 999 9),最低定量限为0.2 μg•ml-1，方法回收率99.28%～101.38%，提取回收率 97.18%～107.28%，日内和日间RSD均小于10%。结论：本法简便、快捷、灵敏,适用于非诺贝特药物动力学研究。
关键词 非诺贝特; 非诺贝特酸; 高效液相色谱法; 药动学
中图分类号：R939.1
文献标识码：A
文章编号：1008-049X(2007)06-0530-03
Determination of Bioactive Metabolite Fenofibric Acid of Fenofibrate in Human Plasma by HPLC
Xu Fan, Feng Enfu,YU Fang(Kunming General Hospital of Chengdu Military Region, Yunnan, Kunming 650032,China)
ABSTRACT Objective: To establish an HPLC method to determine the concentration of bioactive metabolite fenofibric acid offenofibrate. Method: Plasma protein was precipitated by methanol; Waters sunfire C18 column(150 mm×4.6 mm,5 μm) was used; the mobile phase was0.05 mol•L-1,potassium dihydrogen phosphatemethanol(70∶30, adjust pH to 2.5 with phosphoric acid).The detection wavelength was at 288 nm. Result: The retention time of fenofibric acid was 6.7 minute. The limit of quantitation for fenofibric acid was 0.2 μg•ml-1.RSDsmaller than 20%.It was well linearity in the range from 0.2 μg•ml-1to 20.0μg•ml-1. Conclusion: The method was convenient,fast and sensitive.
KEY WORDS Fenofibrate;Fenofibric acid;HPLC;Pharmacokinetics
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