苗军 (吉林省第四医院药剂科 长春 130012) 张馨木 张赢予 (吉林大学再生医学研究所生化室）
摘 要 目的：探讨乌司他汀预处理对肝脏缺血再灌注损伤的保护作用。方法：取大鼠80只,随机分为0.9%氯化钠溶液预处理和乌司他汀预处理两组,观察肝脏缺血再灌注损伤引起的变化及抑肽酶给药后的影响。结果：乌司他汀给药组大鼠缺血再灌注损伤引起的血清ALT 、AST活性及肝组织丙二醛(MDA) 含量变化均显著低于0.9%氯化钠溶液组(P<0.01);肝细胞形态学异常改变也明显较0.9%氯化钠溶液预处理组轻。结论：乌司他汀预处理对大鼠肝脏缺血再灌注损伤有明显的防治作用。
关键词 乌司他汀;肝脏;缺血再灌注损伤;丙二醛
中图分类号：R657.3
文献标识码：A
文章编号：1008-049X(2007)06-0523-03
Experimental Study of Ulinastatin Protecting the Liver from Ischemia reperfusion Injury in Rats
Miao Jun(（Fourth People Hospital of Jilin Province,Changchun 130012, China); Zhang Xinmu, Zhang Yingyu(Department of Biochemistry, Insistute of Frontier Medical Sciences ,Jilin University）
ABSTRACT Objective: To investigate the protective effect of ulinastatin pretreatment on the ischemiareperfusion injury in the liver of rats. Method: Eighty adult rats were divided randomly into two groups: normal saline pretreatment group and ulinastatin pretreatment group. The liver changes induced by the ischemiareperfusion and the effect after ulinastatin pretreatment were observed. Result: Compared with group Ⅰ,the activities of serum ALT,AST and the content of MDA decreased significantly(P＜0.01).The morphologic changes of liver tissue showed more severe in GroupⅠ than that in Group Ⅱ both under the optical microscope or under the electronic microscope. Conclusion: With the ulinastatin pretreatment, liver tissue was protected effectly from ischemiareperfusion injury.
KEY WORDS Ulinastatin; Liver; Ischemiareperfusion injury; Malondialdehyde (MDA)
参 考 文 献
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