李碧峰 冯惠平 贾晋蓉 陈青青 (漳州市医院药剂科 福建漳州 363000)
摘 要 目的：建立一种简便可行的RP-HPLC法测定全血中环孢素（CsA）的浓度，为临床合理应用CsA提供参考。方法：全血样品1 ml经乙醚单步提取，正已烷洗涤去干扰后采用C18柱（200 mm×4.6 mm，5 μm）分离；流动相为乙腈-甲醇-水（72∶6∶22）；流速为1.5 ml•min-1；柱温为60℃；检测波长为210 nm；环孢素D（CsD）为内标。结果：CsA全血浓度在50～1 000 ng•ml-1范围内呈良好的线性关系（r=0.999 6），平均方法回收率为96.6%～99.2%，平均萃取回收率为59.5%～65.3%；日内、日间RSD均小于6%，最低检测限为10 ng•ml-1。结论：本法简便、快速、准确可靠，用于移植术后患者及再生障碍性贫血患者的CsA血药浓度监测，效果良好。
关键词 反相高效液相色谱法；环孢素；血药浓度监测
中图分类号：R97
文献标识码：A
文章编号：1008-049X(2007)07-0649-03
Determination of Cyclosporin in Human Whole Blood by RP-HPLC
Li Bifeng，Feng Huiping，Jia Jinrong，Chen Qingqing(Zhangzhou Municipal Hospital，Fujian，Zhangzhou 363000,China)
ABSTRACT Objective: To establish a practical RP-HPLC method to determine the whole blood concentration of cyclosporin A.Method: CsA was extracted from whole blood with diethyl and removed the endogenous interference in blood with nhexane ether and separated.Diamonisi C18column (200 mm×4.6 mm，5 μm) was used；mobile phase was acetonitrilemethanoldeionized water (72∶6∶22)；the temperature of column was set at 60 ℃；The detecting wavelength was 210 nm；the internal standard was cyclosporin D.Result:A good linear relationship was obtained between the amount of CsA from 50 ng•ml-1to 1 000 ng•ml-1(r=0.999 6).The average method recovery rate of CsA was 96.6%-99.2%，the average extraction recovery rate was 59.5%-65.3% and the RSD with inday and betweenday were less than 6%.The detection limit of CsA was 10 ng•ml-1.Conclusion: The method is accurate，simple，sensitive and suitable for the monitoring blooddrug concentration of CsA after transplantation and aplastic anemia.
KEY WORDS RP-HPLC；Cyclosporin A；Therapeutic drug monitoring
参 考 文 献
1 丁俊杰，李中东，焦正，等.人全血中环孢素A浓度的HPLC测定及药物动力学研究［J］.中国医药工业杂志，2004，35 (2)：91-93
2 任斌，黎曙霞，陈孝，等.反相高效液相色谱法测定环孢素A血药浓度［J］.中国医院药学杂志，2001，21(8)：470-472
3 徐霞，纪松岗，刘丽岗，等.反相高效液相色谱法测定全血中环孢素A的浓度［J］.解放军药学学报，2002，18(2)：116-118 资料来自:中国药师-专业站 |