郭胜才 徐兰英 邹晓华 (解放军第117医院 杭州 310013)
摘 要 目的：建立法莫替丁的含量测定方法，控制法莫替丁制剂的质量。方法：采用高效液相色谱法，以ODS为固定相，庚烷磺酸钠溶液乙腈(80∶20)为流动相，用外标法计算含量。结果：法莫替丁在20.0～160.0μg.ml-1范围内呈良好线性关系，r=0.999 7，平均回收率为100.4%，日内和日间精密度分别为1.1%和0.7%。结论：本方法操作方便，测定结果准确，可用于注射用法莫替丁的含量测定及质量控制。
关键词 法莫替丁；高效液相色谱法；含量测定
中图分类号：R927.2
文献标识码：A
文章编号：1008-049X(2007)09-0889-02
Determination of Famotidine in Famotidine and Sodium Chloride Injection by HPLC
Guo Shengcai, Xu Lanying, Zou Xiaohua (Department of pharmacy, No.117th Hospital, PLA， Hangzhou 310013, China)
ABSTRACT Objective: To establish a method to determine the famotidine in famotidine and sodium chloride injection for quality control.
Method: The MERCK LiChrospher100 C18column(125 mm×4.6 mm,5 μm) was used,the mobile phase consisted of sodium heptanesulfonate solutionacetonitrile(80∶20),the wavelength was at 254 nm.The content of famotidine was determined by external standard method.
Result: The calibration curve was in good linearity within the range of 20.0to 160.0μg.ml-1(r=0.999 7.) The average recovery of famotidine was 100.4%,the innerday and betweenday RSDwas 1.1%and 0.7%, respectively.Conclusion: This method is simple, rapid, accurate and reproducible, and can be used for determination and quality control of famotidine and sodium chloride injection.
KEY WORDS Famotidine; HPLC; Determination
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