夏东亚1 许霁虹2 王彦1 张铁铮2 (沈阳军区总医院1.药剂科，2.麻醉科 沈阳 110016)
摘 要 目的：建立同时测定丙泊酚和硫喷妥钠含量的高效液相色谱方法，观察丙泊酚与硫喷妥钠混合液中两成分含量的稳定性。方法：采用Lichrosorb C18色谱柱（200 mm×4.6 mm, 5 μm），甲醇-水（70∶30）为流动相，流速1.0 ml.min-1；检测波长222 nm，以麝香草酚为内标。将1%丙泊酚与2.5%硫喷妥钠1∶1混合液及1%丙泊酚注射液、2.5%硫喷妥钠注射液分别置冰箱4 ℃，室温25 ℃和恒温箱40℃环境中。在第0，1，3，7天分别取样，测定丙泊酚和硫喷妥钠的含量。结果：丙泊酚和硫喷妥钠的线性范围分别为10～60 μg.ml-1（r＝0.999 7）和25～125 μg.ml-1（r＝0.999 6），平均回收率分别为99.8%和100.7%，RSD分别为 2.4%和1.3％。1%丙泊酚与 2.5%硫喷妥钠1∶1混合液在 4 ℃和25 ℃条件下放置1，3，7 d，丙泊酚和硫喷妥钠含量下降均小于10%，在40 ℃条件下放置3，7 d，硫喷妥钠含量下降明显。结论：同时测定丙泊酚和硫喷妥钠含量的方法简便，准确。1%丙泊酚与2.5%硫喷妥钠1∶1混合液在4 ℃和25 ℃条件下稳定。
关键词 丙泊酚；硫喷妥钠；高效液相色谱法；含量稳定性
中图分类号：R942
文献标识码：A
文章编号：1008-049X(2007)09-0881-03
Determination of Propofol and Thiopental in a 1∶1 Volume Mixture by HPLC and Its Chemical Stability
Xia Dongya1, Xu Jihong2, Wang Yan1, Zhang Tiezheng2(1.Department of Pharmacy，General Hospital of Shenyang Military Region, Shenyang 110016, China； 2. Department of Anesthesiology)
ABSTRACT Objective: To establish a method for the simultaneous determination of propofol and thiopental by HPLC, and study the stability of propofol and thiopental mixture.Method: The Lichrosorb C18 column (200 mm×4.6 mm, 5 μm)was used. The mobile phase was methanolwater (70∶30).The flow rate was 1.0ml.min-1.The detection wavelength was at 222 nm. The internal standard was thymol. The 1% propofol and 2.5% thiopental in a 1∶1 volume mixture was maintained at 4 ℃,25 ℃ and 40 ℃, respectively. The propofol and thiopental concentrations of samples were measured at time of 0 ,1, 3, and 7 days.Result: The linear rang of propofol and thiopental was 10～60 μg.ml-1(r＝0.999 7) and 25～125 μg.ml-1(r＝0.999 6), respectively. The average recovery were 99.8%and 100.7% with RSD2.4%and 1.3%,respectively.The propofol and thiopental concentrations of samples have no significant changes at 4 ℃, 25 ℃ in 7 days.Conclusion: The method appeared to be simple, accurate.The 1% propofol and 2.5%thiopental in a 1∶1 volume mixture at 4 ℃, 25 ℃ was stable.
KEY WORDS Propofol; Thiopental; HPLC; Content stability
参 考 文 献
1 陈新谦，金有豫，汤光.新编药物学［M］.第15版.北京：人民卫生出版社，2003.293
2 Lazar ER,Jollyd DT, Tam TK,et al.Propofol and thiopental in a 1∶1 volume mixture is chemically stable［J］.Anesth Analg, 1998,86: 422-426 资料来自:中国药师-专业站 |