郑芳 朱雪松 (郧阳医学院附属东风总医院药剂部 湖北十堰 442008)
摘 要 目的：建立氧氟沙星氢化可的松滴耳液含量测定的方法。方法：采用RP-HPLC法测定滴耳液中氧氟沙星与氢化可的松的含量。色谱柱为Poresphere C18(150 mm×4.6 mm,5 μm)；流动相为甲醇-0.025 mol.L-1磷酸溶液（用三乙胺调pH至3.0）（55∶45）; 流速0.8 ml.min-1; 检测波长为240 nm; 检测灵敏度为0.16AUFS，进样量20 μl。结果：氧氟沙星在30~480 μg.ml-1浓度范围内，峰面积与浓度的线性关系良好（r=0.999 9），平均回收率为99.8%; 氢化可的松在5~80 μg.ml-1浓度范围内，峰面积与浓度的线性关系良好（r=0.999 9），平均回收率为 99.9%。结论：本方法快速、简便，精密度好，重复性好，可作为氧氟沙星氢化可的松滴耳液的含量测定方法。
关键词 氧氟沙星；氢化可的松；含量测定；RP-HPLC
中图分类号：R927.2
文献标识码：A
文章编号：1008049X(2007)09-0877-02
Determination the Content of Ofloxacin Hydrocortisone Ear Drops by RP-HPLC
Zheng Fang，Zhu Xuesong（Department of Pharmacy,General Hospital of Dong Feng Motor Corporation,Hubei，Shiyan 442008，China）
ABSTRACT Objective: To determine the content of ofloxacin hydrocortisone ear drops.Method: RP-HPLC method was adopted to determine ofloxacin and hydrocortisone simultaneously.The Poresphere C18column (150 mm×4.6 mm,5 μm)was used, mobile phase consisted of methanol0.025 mol.L-1 phosphic acid solution（adjusted to pH3.0with triethylamine）（55∶45）, with flow rate of 0.8 ml.min-1 and detection wavelength was at 240 nm. The detection sensitivity was 0.16AUFS and the injected volume was 20 μl.
Result: The calibration curve of ofloxacin was linear within the range of 30 to 480 μg.ml-1（r=0.999 9） and the average recovery was 99.8%.The calibration curve of hydrocortisone was linear within the range of 5 to 80 μg.ml-1（r=0.999 9） and the average recovery was 99.9%. Conclusion: The method is simple, rapid, accurate and reproducible, and can be used for quality control of the preparation.
参 考 文 献
1 中国药典［S］.2005年版.二部.402-403
2 贾淑洁，张蓉，刘美欣，等.RP-HPLC法测定得方氧氟沙星滴眼液中氧氟沙得的含量［J］.中国药房，2002，13（2）：109-110
资料来自:中国药师-专业站 |