孙业成 （吉林北华大学医学院附属医院 吉林吉林 132001） 李亚荣 程晓英 （吉林市食品药品检验所）
摘 要 目的：建立牛黄清火丸的含量测定方法。方法：采用高效液相色谱法。色谱柱：SHIMADZU VP-ODS（250 mm×4.6 mm,5 μm），流动相：甲醇-0.1%磷酸溶液（80∶20，V/V），流速：1.0 ml.min-1，检测波长：254 nm，柱温：30 ℃。结果：大黄素在10.04～50.20 μg.ml-1范围内线性关系良好（r=0.999 8）；平均加样回收率为98.0%，RSD=1.5%（n=6）；大黄酚在20.04～60.12 μg.ml-1范围内线性关系良好（r=1.0000）；平均加样回收率为99.2%，RSD1.1%（n=6）。结论：该方法简便易行，结果准确可靠，可适用于牛黄清火丸的质量控制。
关键词 高效液相色谱法；牛黄清火丸；大黄素；大黄酚；含量测定
中图分类号：R927.2
文献标识码：A
文章编号：1008-049X(2007)09-0873-02
Determination the content of Emodin and Chrysophanol in Niuhuangqinghuo pills by HPLC
Sun Yecheng(Affiliated Hospital, Medical college of Jilin Beihua University, Jilin Jilin 132001,China); Li Yarong,Cheng Xiaoying(Jilin Institute for Food and Drug Control)
ABSTRACT Objective: To establish a HPLC method for determination the content of emodin and chrysophanol in Niuhuangqinghuo pills.Method: The shimadzu VP-ODS C18column(250 mm×4.6 mm,5 μm) was used, the mobile phase was methanol0.1%phosphoric acid solution(80∶20),the column temperature was 30 ℃,the detection wavelength was 254 nm.the flow rate was 1.0 ml.min-1.
Result: The linear ranges of emodin and chrysophanol were at 10.04-50.20 μg(r=0.999 8) and 20.4-60.12 μg.ml-1(r=1.000 0) respectively, the average recoveries(n=6) were 98.0% and 99.2%withRSD1.5%and 1.1% respectivery.Conclusion: The method is simple and accurate, it can be used for quality control of Niuhuangqinghuo pills.
KEY WORDS HPLC; Niuhuangqinghuo pills; emodin;chrysophanol; determination
参 考 文 献
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2 中国药典［S］. 2005年版.一部.613
3 原永芳,刘爱波，金山丛，等.新脂康口服液中大黄素和大黄酚的HPLC法定量分析［J］.中国药师, 2004,12（12）:940-942 资料来自:中国药师-专业站 |