于玲 王晓飞 汪杰 赵康虎 (武警部队药品仪器检验所 北京 102613)
摘 要 目的：建立以高效液相色谱法测定肾舒颗粒中盐酸小檗碱含量的方法。方法：色谱柱：资生堂ODS C18，流动相：乙腈-磷酸二氢钠（0.025 mol•L-1）-十二烷基硫酸钠（0.025 mol•L-1）（50∶25∶25），柱温：25 ℃，检测波长：365 nm，流速：1.0 ml•min-1。结果：盐酸小檗碱进样量在0.19～1.9 μg范围内线性良好(r=0.999 9)，平均回收率：99.34%,RSD 1.76%（n=6）。结论：本方法简便，准确、重复性好,可用于该制剂的质量控制。
关键词 高效液相色谱法;肾舒颗粒;黄柏;盐酸小檗碱;含量测定
中图分类号：R927.2
文献标识码：A
文章编号：1008-049X(2007)10-0996-02
Determination the Content of Berberine Hydrochloride in Shenshu granules by HPLC
Yu Ling，Wang Xiaofei，Wang Jie，Zhao Kanghu (Institute for Drug Control of the Chinese People 's Armed Police Forces , Beijing 102613,China)
ABSTRACT Objective: To establish an HPLC method for determination the content of berberine hydrochloride in Shenshu granules. Method:The SHISEIDO ODS C18 column was used;mobile phase was acetonitrilesodium dihydrogen phosphate（0.025mol•L-1）sodium dodecyl sulfate（0.025mol•L-1）（50∶25∶25）;column temperature was 25 ℃;detection wavelength was at 365 nm;flow rate was 1.0 ml•min-1.Result:The calibration curve of berberine hydrochloride had a good linear relationship in the range of 0.19-1.9μg (r=0.999 9),the average recovery was99.34%,the RSDwas1.76%（n=6）.Conclusion: The method is simple, accurate and reproductive. It can be used for quality control of this preparation.
KEY WORDS HPLC；Shenshu granules；Cortex phellodendri,；Berberine hydrochloride;Content Determination
参 考 文 献
1 中华人民共和国卫生部药品标准［S］.中药成方制剂第九册.91
2 刘腊娥，陈立新，才亚民，等.高效液相色谱法测定肾康口服液中盐酸小檗碱的含量［J］.中国药房，2005，16(2)：137-138
3 王敏，李翔，王洪，等.反相离子对高效液相色谱法测定黄柏及其颗粒剂中小檗碱及巴马汀的含量［J］.第二军医大学学报， 2005，26（2）：195-197 资料来自:中国药师-专业站 |